DeepBtoD: Improved RNA-binding proteins prediction via integrated deep learning.

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Abstract

RNA-binding proteins (RBPs) have crucial roles in various cellular processes such as alternative splicing and gene regulation. Therefore, the analysis and identification of RBPs is an essential issue. However, although many computational methods have been developed for predicting RBPs, a few studies simultaneously consider local and global information from the perspective of the RNA sequence. Facing this challenge, we present a novel method called DeepBtoD, which predicts RBPs directly from RNA sequences. First, a [Formula: see text]-BtoD encoding is designed, which takes into account the composition of [Formula: see text]-nucleotides and their relative positions and forms a local module. Second, we designed a multi-scale convolutional module embedded with a self-attentive mechanism, the ms-focusCNN, which is used to further learn more effective, diverse, and discriminative high-level features. Finally, global information is considered to supplement local modules with ensemble learning to predict whether the target RNA binds to RBPs. Our preliminary 24 independent test datasets show that our proposed method can classify RBPs with the area under the curve of 0.933. Remarkably, DeepBtoD shows competitive results across seven state-of-the-art methods, suggesting that RBPs can be highly recognized by integrating local [Formula: see text]-BtoD and global information only from RNA sequences. Hence, our integrative method may be useful to improve the power of RBPs prediction, which might be particularly useful for modeling protein-nucleic acid interactions in systems biology studies. Our DeepBtoD server can be accessed at http://175.27.228.227/DeepBtoD/.