Deepening the role of excitation/inhibition balance in human iPSCs-derived neuronal networks coupled to MEAs during long-term development.
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Abstract

The purpose of this study is to investigate whether and how the balance between excitation and inhibition (‘E/I balance’) influences the spontaneous development of human-derived neuronal networks in vitro. To achieve that goal, we performed a long-term (98 days) characterization of both homogeneous (only excitatory or inhibitory neurons) and heterogeneous (mixed neuronal types) cultures with controlled E/I ratios (i.e., E:I 0:100, 25:75, 50:50, 75:25, 100:0) by recording their electrophysiological activity using Micro-Electrode Arrays (MEAs).Excitatory and inhibitory neurons were derived from human induced pluripotent stem cells (h-iPSCs). We realized five different configurations by varying the glutamatergic and GABAergic percentages.
Main results.
We successfully built both homogeneous and heterogeneous cultures finely controlling the E/I ratios and we were able to maintain them for up to 3 months. Homogeneity differentially impacted purely inhibitory (no bursts) and purely excitatory (few bursts) networks, deviating from the typical traits of heterogeneous cultures (burst dominated). Increased inhibition in heterogeneous cultures strongly affected the duration and organization of bursting and network bursting activity. Connectivity and deep learning-based analysis further confirmed all the above.Healthy neuronal activity is controlled by a well-defined E/I balance whose alteration could lead to the onset of neurodevelopmental disorders like schizophrenia or epilepsy. Most of the commonly used in vitro models are animal-derived or too simplified and thus far from the in vivo human condition. In this work, by performing a long-term study of h-iPSCs-derived neuronal networks obtained from healthy human subjects, we demonstrated the feasibility of a robust in vitro model which can be further exploited for investigating pathological conditions where the E/I balance is impaired.
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