Automated Stain-Free Histomorphometry of Peripheral Nerve by Contrast-Enhancing Techniques and Artificial Intelligence.

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Abstract

Traditional histopathologic evaluation of peripheral nerve using brightfield microscopy is resource-intensive, necessitating complex sample preparation. Label-free imaging techniques paired with artificial intelligence-based image reconstruction and segmentation may facilitate peripheral nerve histomorphometry.Herein, the utility of label-free phase contrast techniques paired with artificial intelligence-based image processing for imaging of mammalian peripheral nerve is demonstrated.Fresh frozen murine sciatic nerve sections were imaged in transmission modalities using differential interference and phase contrast microscopy and in epifluorescent modality following staining with myelin-specific dye. Deep learning was employed to predict epifluorescent images from transmitted phase contrast images, and machine learning employed for automated segmentation of myelinated axons for reporting of axons counts and g-ratios.Conventional peripheral nerve histomorphometry is resource intensive.Clinical applications of label-free phase contrast microscopy paired with deep learning algorithms are discussed.Copyright © 2022. Published by Elsevier B.V.